Search for and analysis of single nucleotide polymorphisms (SNPs) in rice (Oryza sativa, Oryza rufipogon) and establishment of SNP markers

被引：153

作者：

Nasu, S ^{[1
]}

Suzuki, J ^{[1
]}

Ohta, R ^{[1
]}

Hasegawa, K ^{[1
]}

Yui, R ^{[1
]}

Kitazawa, N ^{[1
]}

Monna, L ^{[1
]}

Minobe, Y ^{[1
]}

机构：

[1] Plant Genome Ctr, Tsukuba, Ibaraki 3050856, Japan

来源：

DNA RESEARCH | 2002年 / 9卷 / 05期

关键词：

rice (Oryza sativa L; Oryza rufipogon); single nucleotide polymorphism (SNP); molecular marker; genome sequence; polymerase chain reaction (PCR);

D O I：

10.1093/dnares/9.5.163

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We searched for SNPs in 417 re-ions distributed throughout the genome of three Oryza sativa ssp. japonica cultivars, two indica cultivars, and a wild rice (O. ryfipogon). We found 2800 SNPs in approximately 250,000 aligned bases for an average of one SNP every 89 bp, or one SNP every 232 bp between two randomly selected strains. Graphic representation of the frequency of SNPs along each chromosome showed uneven distribution of polymorphism-rich and -poor regions, but little obvious association with the centromere or telomere. The 94 SNPs that we found between the closely related cultivars 'Nipponbare' and 'Koshihikari' can be converted into molecular markers. Our establishment of 213 co-dominant SNP markers distributed throughout the genome illustrates the immense potential of SNPs as molecular markers not only for genome research, but also for molecular breeding of rice.

引用

页码：163 / 171

页数：9

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