An internal control for the diagnosis of crown gall by PCR

被引：24

作者：

Cubero, J

van der Wolf, J

van Beckhoven, J

López, MM

机构：

[1] IVIA, Dept Protecc Vegetal & Biotecnol, Moncada 46113, Valencia, Spain

[2] Plant Res Int, NL-6700 AA Wageningen, Netherlands

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2002年 / 51卷 / 03期

关键词：

Agrobacterium; detection; PCR; internal control;

D O I：

10.1016/S0167-7012(02)00153-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown call diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the attainment of at least one amplification product in every PCR reaction if the DNA extracted is of high enough quality to be amplified. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：387 / 392

页数：6

共 32 条

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