Fluorescent transformation markers for insect transgenesis

被引：115

作者：

Horn, C ^{[1
]}

Schmid, BGM ^{[1
]}

Pogoda, FS ^{[1
]}

Wimmer, EA ^{[1
]}

机构：

[1] Univ Bayreuth, Lehrstuhl Genet, D-95447 Bayreuth, Germany

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2002年 / 32卷 / 10期

关键词：

green fluorescent protein; EGFP; ECFP; EYFP; DsRed; germ-line transformation; transposable elements; transposon mutagenesis; enhancer trapping;

D O I：

10.1016/S0965-1748(02)00085-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The first effectively achieved germ-line transformations of non-drosophilid insects were based on mutant rescue of eye color phenotypes. However, for most insect species neither visible mutants nor corresponding cloned genes are available. Therefore, the development of broadly applicable and reliable transformation markers will be of great importance to fully exploit the enormous potential transgenic insect technology has to offer. Here we review transposon-mediated germ-line transformation approaches that employ green fluorescent protein (GFP) variants to identify successful gene transfer. Furthermore, we provide novel data on the use of DsRed as an additional red fluorescent transformation marker for insect transgenesis. In conclusion, fluorescent proteins controlled by suitable strong promoters possess ideal characteristics to serve as transformation markers for a wide range of insect species. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1221 / 1235

页数：15

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