Determination of the stability constants of inclusion complexes of p-H-37-(2-carboxy-methyloxy)-calix-[6]-arene and p-sulphonato-37-(2-carboxy-methyloxy)-calix-[6]-arene with 15 amino acids by RP-HPLC

Reversed-phase high performance liquid chromatography ( Separon SGX CN, UV detection at 254 nm and water as mobile phase) was applied to the study of the host-guest complexation of p-H-37-(2-carboxy-methyloxy)-calix-[6]-arene (1) and p-sulphonato-37-(2-carboxy-methyloxy)-calix-[6]-arene (2) with 15 amino acids in the mobile phase. It was established that the formation of the inclusion complexes results in changes in the retention times and capacity factors of amino acids. Stability constants of the complexes were determined. The variations in stability constant values may be explained in terms of the different interactions, which may occur between amino acids and 1 and 2.