A 3D-impedimetric immunosensor based on foam Ni for detection of sulfate-reducing bacteria

被引:52
作者
Wan, Yi [1 ,2 ]
Zhang, Dun [1 ]
Wang, Yi [1 ]
Hou, Baorong [1 ]
机构
[1] Chinese Acad Sci, Key Lab Corros Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
基金
中国国家自然科学基金;
关键词
3D-immunosensor; Sulfate-reducing bacteria; Foam Ni; Electrochemical impedance spectroscopy; SALMONELLA-TYPHIMURIUM; IMPEDIMETRIC IMMUNOSENSOR;
D O I
10.1016/j.elecom.2009.12.017
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
A 3D-immunosensor based on simple and efficient trapping platform (foam Ni) combining with adsorption of gold nanoparticles and specific recognition of biological/chemical molecular has been reported for detection of sulfate-reducing bacteria (SRB) using electrochemical impedance spectroscopy (EIS). The impedance spectra were also used to characterize the successful construct and stepwise modification of the impedimetric immunosensors. This results show that a linear relationship between electron-transfer resistance (R-ct) values and the logarithm of the SRB concentrations was obtained for the SRB concentration range of 2.1 x 10(1)-2.1 x 10(7) cfu/ml. Additionally, the fabricated immunosensor shows a high selectivity against other bacteria. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:288 / 291
页数:4
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