Improved 16S rRNA-targeted probe set for analysis of sulfate-reducing bacteria by fluorescence in situ hybridization

被引：95

作者：

Luecker, Sebastian

Steger, Doris

Kjeldsen, Kasper Urup

MacGregor, Barbara J.

Wagner, Michael

Loy, Alexander

机构：

[1] Univ Vienna, Dept Mikrobielle Okol, Fak Lebenswissensch, A-1090 Vienna, Austria

[2] Univ Aarhus, Inst Biol Sci, Dept Microbiol, DK-8000 Aarhus C, Denmark

[3] Univ N Carolina, Dept Marine Sci, Chapel Hill, NC 27599 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 69卷 / 03期

基金：

奥地利科学基金会;

关键词：

fluorescence in situ hybridization; Deltoproteobacteria; sulfate-reducing bacteria; 16S rRNA-targeted probes;

D O I：

10.1016/j.mimet.2007.02.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An updated dataset of in silico specificities for 54 previously published 16S rRNA-targeted oligonucleotides was assembled to provide guidance for reliable fluorescence in situ hybridization (FISH) analysis of sulfate-reducing bacteria. Additionally, six new FISH probes were developed for major deltaproteobacterial taxa, including a probe trio targeting most Deltaproteobacteria and Gemmatimonadetes. (C) 2007 Elsevier B.V. All rights reserved.

引用

收藏

页码：523 / 528

页数：6

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