Effect of static pressure on human granulocyte-macrophage stimulating factor (hGM-CSF) production by Chinese hamster ovary cells

被引：5

作者：

Gong, H ^{[1
]}

Takagi, M ^{[1
]}

Moriyama, T ^{[1
]}

Ohno, T ^{[1
]}

Yoshida, T ^{[1
]}

机构：

[1] Osaka Univ, Int Ctr Biotechnol, Suita, Osaka 5650871, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2002年 / 94卷 / 03期

关键词：

static pressure; CHO cells; GM-CSF; protein production; mRNA;

D O I：

10.1263/jbb.94.271

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of static pressure on the production of human granulocyte-macrophage colony stimulating factor (hGM-CSF) by the Chinese hamster ovary (CHO) cell line DR1000L4N was investigated. Although constant static pressures between 0.15 and 0.9 MPa had little influence on specific growth rate, the specific production rate of hGM-CSF increased from 1.23 to 1.62x10(-13) mg/cell/h in proportion to static pressure. The specific rate of tPA production by the human embryo lung cell line MRC-5 also increased from 2.92 to 6.38 x 10(-12) mg/cell/h as static pressure increased. The expression level of hGM-CSF mRNA increased with the pressurization even for 4 h.

引用

页码：271 / 274

页数：4