Production of lactic acid from pulp mill solid waste and xylose using Lactobacillus delbrueckii (NRRL B445)

被引:22
作者
Thomas, S [1 ]
机构
[1] Auburn Univ, Dept Chem Engn, Auburn, AL 36849 USA
关键词
xylose; pulp mill solid waste; lactic acid; Lactobacillus fermentation;
D O I
10.1385/ABAB:84-86:1-9:455
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using the simultaneous saccharification and fermentation (SSF) technique, pulp mill solid waste cellulose was converted into glucose using cellulase enzyme and glucose into lactic acid using NRRL B445. SSF experiments were conducted at various pH levels, temperatures, and nutrient concentrations, and the lac tic acid yield ranged from 86 to 97%. The depletion of xylose in SSF was further investigated by inoculating NRRL B445 into a xylose-only medium. On prolonged incubation, depletion of xylose with lactic acid production was observed. An experimental procedure with a nonglucose medium was developed to eliminate the lag phase. From xylose fermentation, Lactobacillus delbrueckii yielded 88-92% lactic acid and 2-12% acetic acid.
引用
收藏
页码:455 / 468
页数:14
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