Molecular characterization of the genetic integrity of wheat (Triticum aestivum L.) germplasm after long-term maintenance

被引：62

作者：

Börner, A

Chebotar, S

Korzun, V

机构：

[1] IPK, D-06466 Gatersleben, Germany

[2] Plant Breeding & Genet Inst, UA-270036 Odessa, Ukraine

来源：

THEORETICAL AND APPLIED GENETICS | 2000年 / 100卷 / 3-4期

关键词：

ex-situ conservation; genebank collection; long term storage; mikrosatellites; wheat;

D O I：

10.1007/s001220050064

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The genetic identity of eight wheat (Triticum aestivum L.) accessions maintained in the Gatersleben genebank and regenerated up to 24 times was studied by using wheat microsatellite markers (WMS). It was demonstrated that WMS can be used to analyze bulks of seeds stored more than 50 years in a seed reference collection at room temperature. No contamination due to foreign pollen or incorrect handling during the multiplication cycles was discovered. For one accession (TRI 4599) genetic drift was observed, whereas for TRI 249 a heterogenous situation for two markers was maintained over the years. We were able to show that microsatellites can be used as a simple and reliable marker system for the verification of the integrity and genetic stability of genebank accessions.

引用

页码：494 / 497

页数：4

共 11 条

[1]

[Anonymous], 1996, STAT WORLDS PLANT GE

[2]

[Anonymous], 1996, REP STAT WORLDS PLAN

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