Fail-Safe Transcriptional Termination for Protein-Coding Genes in S. cerevisiae

被引：88

作者：

Rondon, Ana G. ^{[1
]}

Mischo, Hannah E. ^{[1
]}

Kawauchi, Junya ^{[1
]}

Proudfoot, Nick J. ^{[1
]}

机构：

[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England

来源：

MOLECULAR CELL | 2009年 / 36卷 / 01期

基金：

英国惠康基金;

关键词：

RNA-POLYMERASE-II; CRYPTIC UNSTABLE TRANSCRIPTS; SMALL NUCLEOLAR RNAS; MESSENGER-RNA; SACCHAROMYCES-CEREVISIAE; 3'-END FORMATION; HELICASE SEN1P; YEAST; EXOSOME; COMPLEX;

D O I：

10.1016/j.molcel.2009.07.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcription termination of RNA polymerase II (Pol II) on protein-coding genes in S. cerevisiae relies on pA site recognition by 3' end processing factors. Here we demonstrate the existence of two alternative termination mechanisms that rescue polymerases failing to disengage from the template at pA sites. One of these fail-safe mechanisms is mediated by the NRD complex, similar to termination of short non-coding genes. The other termination mechanism is mediated by Rnt1 cleavage of the nascent transcript. Both fail-safe termination mechanisms trigger degradation of readthrough transcripts by the exosome. However, Rnt1-mediated termination can also enhance the usage of weak pA signals and thereby generate functional mRNA. We propose that these alternative Pof II termination pathways serve the dual function of avoiding transcription interference and promoting rapid removal of aberrant transcripts.

引用

页码：88 / 98

页数：11

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