Clinical resistance to erythromycin and clindamycin in cutaneous propionibacteria isolated from acne patients is associated with mutations in 23S rRNA

被引:90
作者
Ross, JI [1 ]
Eady, EA [1 ]
Cove, JH [1 ]
Jones, CE [1 ]
Ratyal, AH [1 ]
Miller, YW [1 ]
Vyakrnam, S [1 ]
Cunliffe, WJ [1 ]
机构
[1] GEN INFIRM,DEPT DERMATOL,LEEDS LS1 3EX,W YORKSHIRE,ENGLAND
关键词
D O I
10.1128/AAC.41.5.1162
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genetic basis of erythromycin resistance in cutaneous propionibacteria was determined by comparing the nucleotide sequences of the peptidyl transferase region in the 23S rRNAs from 9 susceptible and 26 resistant clinical isolates as well as 4 laboratory-selected erythromycin-resistant mutants of a susceptible strain. In 13 isolates and the 4 laboratory mutants, cross-resistance to macrolides, lincosamides, and B-type streptogramins was associated with an A --> G transition at a position cognate with Escherichia coli 23S rRNA base 2058, These strains were resistant to greater than or equal to 512 mu g of erythromycin per ml. Two other mutations were identified, an A --> G transition at base 2059 in seven strains, associated with high-level resistance to all macrolides, and a G --> A transition at base 2057 in six strains, associated with low-level resistance to erythromycin, These mutations correspond to three of four phenotypic classes previously identified by using MIC determinations.
引用
收藏
页码:1162 / 1165
页数:4
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