Characterization of a C-terminal-type kinesin-related protein from Dictyostelium discoideum

被引：7

作者：

Iwai, S ^{[1
]}

Suyama, E ^{[1
]}

Adachi, H ^{[1
]}

Sutoh, K ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Meguro Ku, Tokyo 1538902, Japan

来源：

FEBS LETTERS | 2000年 / 475卷 / 01期

关键词：

kinesin-related protein; C-terminal-type kinesin; mitotic spindle; Dictyostelium discoideum;

D O I：

10.1016/S0014-5793(00)01619-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have determined the full sequence of K2, a kinesin-related protein (KRP) in Dictyostelium discoideum. Sequence homology and domain organization placed K2 in the ncd/Kar3 subfamily of the C-terminal-type KRPs. Bacterially expressed, truncated K2 showed ATP-dependent binding to microtubules and microtubule-stimulated ATPase activity. K2-null cells grew and developed normally, suggesting overlapping functions of K2 with other microtubule motor(s). Overexpression of K2 caused partial mitotic arrest. Green fluorescent protein-tagged full-length K2 localized in the nucleus at the interphase and on the mitotic spindle during mitosis. These results suggest that K2 is a microtubule-dependent motor which may play some roles in mitotic spindles. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：47 / 51

页数：5

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