Adiponectin is stimulated by adrenalectomy in ob/ob mice and is highly correlated with resistin mRNA

被引:64
作者
Makimura, H
Mizuno, TM
Bergen, H
Mobbs, CV
机构
[1] CUNY Mt Sinai Sch Med, Fishberg Res Ctr Neurobiol, Neurobiol Aging Labs, New York, NY 10029 USA
[2] CUNY Mt Sinai Sch Med, Dept Geriatr & Adult Dev, New York, NY 10029 USA
[3] Univ Manitoba, Dept Human Anat & Cell Sci, Winnipeg, MB R3E 0W3, Canada
来源
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 2002年 / 283卷 / 06期
关键词
AdipoQ; adipocyte complement-related protein of 30 kDa; insulin resistance; glucocorticoids; leptin; messenger ribonucleic acid;
D O I
10.1152/ajpendo.00227.2002
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Plasma levels of the adipocyte product adiponectin, a putative insulin-sensitizing agent, are reduced in obesity, whereas plasma levels of resistin, an agent that some believe to confer insulin resistance, are thought to increase with obesity. Because adrenalectomy can increase insulin sensitivity, we hypothesized that adrenalectomy would increase expression of adiponectin and decrease expression of resistin. Therefore, we measured adiponectin mRNA, adiponectin peptide, and resistin mRNA in adrenalectomized ob/ob mice. Adrenalectomy restored adiponectin expression in ob/ob mice to wild-type levels and stimulated adiponectin peptide to above wild-type levels. Surprisingly, expression of adiponectin and resistin was highly positively correlated even after statistical removal of effects of insulin, glucose, and adiposity. In addition, adiponectin and resistin expression were also highly correlated in diet-induced obese mice. The data support a role for adiponectin in mediating some effects of adrenalectomy on insulin sensitivity.
引用
收藏
页码:E1266 / E1271
页数:6
相关论文
共 27 条
[11]   Secretion of adiponectin and regulation of apM1 gene expression in human visceral adipose tissue [J].
Halleux, CM ;
Takahashi, M ;
Delporte, ML ;
Detry, R ;
Funahashi, T ;
Matsuzawa, Y ;
Brichard, SM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 288 (05) :1102-1107
[12]   Adrenalectomy improves diabetes in A-ZIP/F-1 lipoatrophic mice by increasing both liver and muscle insulin sensitivity [J].
Haluzik, M ;
Dietz, KR ;
Kim, JK ;
Marcus-Samuels, B ;
Shulman, GI ;
Gavrilova, O ;
Reitman, ML .
DIABETES, 2002, 51 (07) :2113-2118
[13]   AdipoQ is a novel adipose-specific gene dysregulated in obesity [J].
Hu, E ;
Liang, P ;
Spiegelman, BM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (18) :10697-10703
[14]  
Jonke J, 2002, OBES RES, V10, P1
[15]   CDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (Adipose most abundant gene transcript 1) [J].
Maeda, K ;
Okubo, K ;
Shimomura, I ;
Funahashi, T ;
Matsuzawa, Y ;
Matsubara, K .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 221 (02) :286-289
[16]   Adrenalectomy reverses obese phenotype and restores hypothalamic melanocortin tone in leptin-deficient ob/ob mice [J].
Makimura, H ;
Mizuno, TM ;
Roberts, J ;
Silverstein, J ;
Beasley, J ;
Mobbs, CV .
DIABETES, 2000, 49 (11) :1917-1923
[17]   A transgenic model of visceral obesity and the metabolic syndrome [J].
Masuzaki, H ;
Paterson, J ;
Shinyama, H ;
Morton, NM ;
Mullins, JJ ;
Seckl, JR ;
Flier, JS .
SCIENCE, 2001, 294 (5549) :2166-2170
[18]   Effects of nutritional status and aging on leptin gene expression in mice: Importance of glucose [J].
Mizuno, T ;
Bergen, H ;
Kleopoulos, S ;
Bauman, WA ;
Mobbs, CV .
HORMONE AND METABOLIC RESEARCH, 1996, 28 (12) :679-684
[19]   Obese gene expression: Reduction by fasting and stimulation by insulin and glucose in lean mice, and persistent elevation in acquired (diet-induced) and genetic (yellow agouti) obesity [J].
Mizuno, TM ;
Bergen, H ;
Funabashi, T ;
Kleopoulos, SP ;
Zhong, YG ;
Bauman, WA ;
Mobbs, CV .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (08) :3434-3438
[20]  
Nakano Y, 1996, J BIOCHEM-TOKYO, V120, P803