Comparison of five antibodies as markers in the diagnosis of melanoma in cytologic preparations

被引:73
作者
Sheffield, MV
Yee, H
Dorvault, CC
Weilbaecher, KN
Eltoum, IA
Siegal, GP
Fisher, DE
Chhieng, DC [1 ]
机构
[1] Univ Alabama, Dept Pathol, Birmingham, AL 35249 USA
[2] NYU, Ctr Med, Dept Pathol, New York, NY USA
[3] Lee Pathol Lab, Opelika, AL USA
[4] Washington Univ, Div Oncol, St Louis, MO USA
[5] Univ Alabama, Dept Cell Biol, Birmingham, AL 35294 USA
[6] Univ Alabama, Dept Surg, Birmingham, AL 35294 USA
[7] Dana Farber Canc Inst, Div Pediat Oncol, Boston, MA 02115 USA
[8] Childrens Hosp, Boston, MA 02115 USA
关键词
microphthalmia transcription factor; Melan-A; tyrosinase; S-100; HMB-45; melanoma; immunocytochemistry; cytology;
D O I
10.1092/AU8L65UJFQ75DVGC
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
We determined the sensitivity and specificity of 3 novel antibodies (microphthalmia transcription factor [Mitf] Melan-A, and tyrosinase) as markers for melanoma in cytologic preparations and compared the results with those of commonly used markers (S-100 protein [S-100] and HMB-45). We stained 72 cell blocks from 40 patients with melanoma and 32 with nonmelanocytic malignant neoplasms with antibodies against S-100, HMB-45, Mitf, Melan-A, and tyrosinase. Histologic correlation was available in more than 95% of cases. Nuclear staining for Mitf and cytoplasmic staining for S-100, HMB-45, Melan-A, and tyrosinase in more than 10% of tumor cells was considered positive. All 3 novel markers demonstrated sensitivity superior to S-100 and HMB-45. HMB-45, Melan-A, and Mitf demonstrated specificities of 97%. S-100 protein and tyrosinase were less specific. Sensitivity and specificity for the combination Mitf+/Melan-A+ were 95% and 100%, respectively, whereas they were 80% and 100%, respectively, for S-100+/HMB-45+. Mitf, Melan-A, and tyrosinase are sensitive markers for epithelioid melanoma. Mitf and Melan-A seem more specific than S-100 and tyrosinase. An antibody panel consisting of Mitf and Melan-A is superior to a panel of S-100 and HMB-45 in the diagnosis of melanoma in cytologic specimens.
引用
收藏
页码:930 / 936
页数:7
相关论文
共 58 条
  • [21] Immunohistochemical and reverse transcription-polymerase chain reaction expression analysis of tyrosinase and microphthalmia-associated transcription factor in angiomyolipomas
    Jungbluth, AA
    King, R
    Fisher, DE
    Iversen, K
    Coplan, K
    Kolb, D
    Williamson, B
    Chen, YT
    Stockert, E
    Old, LJ
    Busam, KJ
    [J]. APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, 2001, 9 (01): : 29 - 34
  • [22] T311 - An anti-tyrosinase monoclonal antibody for the detection of melanocytic lesions in paraffin embedded tissues
    Jungbluth, AA
    Iversen, K
    Coplan, K
    Kolb, D
    Stockert, E
    Chen, YT
    Old, LJ
    Busam, K
    [J]. PATHOLOGY RESEARCH AND PRACTICE, 2000, 196 (04) : 235 - 242
  • [23] ROLE OF ANTIBODY TO S100 PROTEIN IN DIAGNOSTIC PATHOLOGY
    KAHN, HJ
    MARKS, A
    THOM, H
    BAUMAL, R
    [J]. AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1983, 79 (03) : 341 - 347
  • [24] ANTIMELANOMA MONOCLONAL ANTIBODY-HMB45 IDENTIFIES AN ONCOFETAL GLYCOCONJUGATE ASSOCIATED WITH IMMATURE MELANOSOMES
    KAPUR, RP
    BIGLER, SA
    SKELLY, M
    GOWN, AM
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1992, 40 (02) : 207 - 212
  • [25] Kaufmann O, 1998, MODERN PATHOL, V11, P740
  • [26] Production of recombinant MART-1 proteins and specific antiMART-1 polyclonal and monoclonal antibodies: Use in the characterization of the human melanoma antigen MART-1
    Kawakami, Y
    Battles, JK
    Kobayashi, T
    Ennis, W
    Wang, X
    Tupesis, JP
    Marincola, FM
    Robbins, PF
    Hearing, VJ
    Gonda, MA
    Rosenberg, SA
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1997, 202 (01) : 13 - 25
  • [27] Microphthalmia transcription factor - A sensitive and specific melanocyte marker for melanoma diagnosis
    King, R
    Weilbaecher, KN
    McGill, G
    Cooley, E
    Mihm, M
    Fisher, DE
    [J]. AMERICAN JOURNAL OF PATHOLOGY, 1999, 155 (03) : 731 - 738
  • [28] Microphthalmia transcription factor and melanoma cell adhesion molecule expression distinguish desmoplastic/spindle cell melanoma from morphologic mimics
    Koch, MB
    Shih, IP
    Weiss, SW
    Folpe, AL
    [J]. AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 2001, 25 (01) : 58 - 64
  • [29] PIGMENTATION GENES - THE TYROSINASE GENE FAMILY AND THE PMEL-17 GENE FAMILY
    KWON, BS
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1993, 100 (02) : S134 - S140
  • [30] Lai R, 1998, CANCER CYTOPATHOL, V84, P92, DOI 10.1002/(SICI)1097-0142(19980425)84:2<92::AID-CNCR4>3.0.CO