Immobilization of Pseudomonas cepacia lipase in a phyllosilicate sol-gel matrix:: effectiveness as a biocatalyst

被引：46

作者：

Hsu, AF ^{[1
]}

Foglia, TA ^{[1
]}

Shen, S ^{[1
]}

机构：

[1] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2000年 / 31卷 / 03期

关键词：

clay; cross-linking; esterification; recycling; tetramethyl orthosilicate;

D O I：

10.1042/BA19990090

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel procedure is described for immobilizing a lipase from Pseudomonas cepacia (PS-30) within a phyllosilicate sol-gel matrix. The method is based on crosslinking a phyllosilicate clay with silicate polymers produced by the controlled hydrolysis of tetramethyl orthosilicate (TMOS). The activity of the phyllosilicate sol-gel-immobilized lipase was dependent upon the type of alkylammonium salt, inorganic catalyst and volume ratio of phyllosilicate clay to TMOS used. Lipase PS-30 immobilized in this way was more stable and had higher activity compared with the free lipase. Studies on the lipase-catalysed esterification of lauric acid with octan-1-ol in iso-octane showed that under controlled water activity conditions the phyllosilicate sol-gel-immobilized lipase had better activity compared with other supported lipase preparations. In addition, the phyllosilicate sol-gel-immobilized lipase was reusable for at least five esterification cycles without significant loss of activity.

引用

页码：179 / 183

页数：5

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