Melting curve analysis of feline calicivirus isolates detected by real-time reverse transcription PCR

被引：55

作者：

Helps, C ^{[1
]}

Lait, P ^{[1
]}

Tasker, S ^{[1
]}

Harbour, D ^{[1
]}

机构：

[1] Univ Bristol, Dept Clin Vet Sci, Div Mol & Cellular Biol, Bristol BS40 5DU, Avon, England

来源：

JOURNAL OF VIROLOGICAL METHODS | 2002年 / 106卷 / 02期

关键词：

FCV; real-time PCR; melting curve;

D O I：

10.1016/S0166-0934(02)00167-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A real-time reverse transcription polymerase chain reaction assay was developed to detect feline calicivirus infection in cats. Using SYBR green I melting curve analysis, isolates could be distinguished by their melting temperature. The assay is sensitive, linear over a wide range of template concentrations and allows accurate quantitation of viral load. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：241 / 244

页数：4

共 11 条

[1] THE COMPLETE NUCLEOTIDE-SEQUENCE OF A FELINE CALICIVIRUS [J].

CARTER, MJ ;

MILTON, ID ;

MEANGER, J ;

BENNETT, M ;

GASKELL, RM ;

TURNER, PC .

VIROLOGY, 1992, 190 (01) :443-448

[2]

COUTTS AJ, 1994, VET REC, V135, P555

[3]

GASKELL RM, 1998, INFECT DIS DOG CAT

[4] Nucleotide sequence of UK and Australian isolates of feline calicivirus (FCV) and phylogenetic analysis of FCVs [J].

Glenn, M ;

Radford, AD ;

Turner, PC ;

Carter, M ;

Lowery, D ;

DeSilver, DA ;

Meanger, J ;

Baulch-Brown, C ;

Bennett, M ;

Gaskell, RM .

VETERINARY MICROBIOLOGY, 1999, 67 (03) :175-193

[5] Use of real-time quantitative PCR to detect Chlamydophila felis infection [J].

Helps, C ;

Reeves, N ;

Tasker, S ;

Harbour, D .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (07) :2675-2676

[6] THE MOLECULAR-CLONING AND SEQUENCE OF AN OPEN READING FRAME ENCODING FOR NONSTRUCTURAL PROTEINS OF FELINE CALICIVIRUS F4 STRAIN ISOLATED IN JAPAN [J].

OSHIKAMO, R ;

TOHYA, Y ;

KAWAGUCHI, Y ;

TOMONAGA, K ;

MAEDA, K ;

TAKEDA, N ;

UTAGAWA, E ;

KAI, C ;

MIKAMI, T .

JOURNAL OF VETERINARY MEDICAL SCIENCE, 1994, 56 (06) :1093-1099

[7] The use of sequence analysis of a feline calicivirus (FCV) hypervariable region in the epidemiological investigation of FCV related disease and vaccine failures [J].

Radford, AD ;

Bennett, M ;

McArdle, F ;

Dawson, S ;

Turner, PC ;

Glenn, MA ;

Gaskell, RM .

VACCINE, 1997, 15 (12-13) :1451-1458

[8] Detection and strain differentiation of feline calicivirus in conjunctival swabs by RT-PCR of the hypervariable region of the capsid protein gene [J].

Sykes, JE ;

Studdert, VP ;

Browning, GF .

ARCHIVES OF VIROLOGY, 1998, 143 (07) :1321-1334

[9] Detection of feline calicivirus, feline herpesvirus 1 and Chlamydia psittaci mucosal swabs by multiplex RT-PCR/PCR [J].

Sykes, JE ;

Allen, JL ;

Studdert, VP ;

Browning, GF .

VETERINARY MICROBIOLOGY, 2001, 81 (02) :95-108

[10] SEQUENCE-ANALYSIS OF THE 3'-END OF FELINE CALICIVIRUS GENOME [J].

TOHYA, Y ;

TANIGUCHI, Y ;

TAKAHASHI, E ;

UTAGAWA, E ;

TAKEDA, N ;

MIYAMURA, K ;

YAMAZAKI, S ;

MIKAMI, T .

VIROLOGY, 1991, 183 (02) :810-814

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