Characterization of a BAHD acyltransferase responsible for producing the green leaf volatile (Z)-3-hexen-1-yl acetate in Arabidopsis thaliana

被引:197
作者
D'Auria, John C.
Pichersky, Eran
Schaub, Andrea
Hansel, Armin
Gershenzon, Jonathan
机构
[1] Max Planck Inst Chem Ecol, Dept Biochem, D-07745 Jena, Germany
[2] Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA
[3] Ionicon Analyt GmbH, A-6020 Innsbruck, Austria
[4] Leopold Franzens Univ Innsbruck, Inst Ionenphys & Angewandte Phys, Innsbruck, Austria
关键词
Arabidopsis thaliana; green leaf volatile; BAHD; acyltransf erase; acetyl coenzyme A; (2)-3-hexen-1-yl acetate; mechanical wounding;
D O I
10.1111/j.1365-313X.2006.02946.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Green-leaf volatiles are commonly emitted from mechanically and herbivore-damaged plants. Derived from the lipoxygenase pathway, these compounds may serve as attractants to predators and parasitoids of herbivores, prevent the spread of bacteria and fungi, and induce several important plant defense pathways. In Arabidopsis thaliana, the major volatile released upon mechanical wounding of the leaves is the GLV ester, (Z)-3-hexen-1-yl acetate. We have characterized a member of the BAHD acyltransferase gene family At3g03480 which catalyzes the formation of (Z)-3-hexen-1-yl acetate from acetyl-CoA and (Z)-3-hexen-1-ol. The encoded acetyl CoA:(Z)-3-hexen-1-ol acetyltransferase (CHAT) has the ability to accept several medium-chain-length aliphatic and benzyl-derived alcohols, but has highest catalytic efficiency with (Z)-3-hexen-1-ol. The highest expression of CHAT occurs in the leaves and stems. Upon mechanical damage, the (Z)-3-hexen-1-yl acetate emission peaked after 5 min and declined to pre-damage levels after 90 min. However, CHAT gene transcript levels increased much more slowly with the highest levels detected between 3 and 6 h after wounding. An increase in CHAT enzyme activity in vitro followed the transcript increase, with levels peaking between 10 and 12 h after wounding. Plants expressing either an RNAi cassette for the CHAT gene or plants harboring a T-DNA insertion in the CHAT coding region had greatly reduced ((Z)-3-hexen-1-yl acetate emission, showing that the CHAT enzyme is responsible for the in planta production of this ester.
引用
收藏
页码:194 / 207
页数:14
相关论文
共 73 条
  • [21] Spatial and temporal analysis of the local response to wounding in Arabidopsis leaves
    Delessert, C
    Wilson, IW
    Van Der Straeten, D
    Dennis, ES
    Dolferus, R
    [J]. PLANT MOLECULAR BIOLOGY, 2004, 55 (02) : 165 - 181
  • [22] EFFECTS OF 6-CARBON ALDEHYDES AND ALCOHOLS ON BACTERIAL PROLIFERATION
    DENG, WL
    HAMILTONKEMP, TR
    NIELSEN, MT
    ANDERSEN, RA
    COLLINS, GB
    HILDEBRAND, DF
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1993, 41 (03) : 506 - 510
  • [23] Variations in CYP74B2 (hydroperoxide lyase) gene expression differentially affect hexenal signaling in the Columbia and Landsberg erecta ecotypes of Arabidopsis
    Duan, H
    Huang, MY
    Palacio, K
    Schuler, MA
    [J]. PLANT PHYSIOLOGY, 2005, 139 (03) : 1529 - 1544
  • [24] Biochemistry of plant volatiles
    Dudareva, N
    Pichersky, E
    Gershenzon, J
    [J]. PLANT PHYSIOLOGY, 2004, 135 (04) : 1893 - 1902
  • [25] Acetyl-CoA:benzylalcohol acetyltransferase -: an enzyme involved in floral scent production in Clarkia breweri
    Dudareva, N
    D'Auria, JC
    Nam, KH
    Raguso, RA
    Pichersky, E
    [J]. PLANT JOURNAL, 1998, 14 (03) : 297 - 304
  • [26] Functional characterization of a melon alcohol acyl-transferase gene family involved in the biosynthesis of ester volatiles.: Identification of the crucial role of a threonine residue for enzyme activity
    El-Sharkawy, I
    Manríquez, D
    Flores, FB
    Regad, F
    Bouzayen, M
    Latché, A
    Pech, JC
    [J]. PLANT MOLECULAR BIOLOGY, 2005, 59 (02) : 345 - 362
  • [27] Airborne signals prime plants against insect herbivore attack
    Engelberth, J
    Alborn, HT
    Schmelz, EA
    Tumlinson, JH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (06) : 1781 - 1785
  • [28] (Z)-3-Hexenol induces defense genes and downstream metabolites in maize
    Farag, MA
    Fokar, M
    Zhang, HA
    Allen, RD
    Paré, PW
    [J]. PLANTA, 2005, 220 (06) : 900 - 909
  • [29] C6-green leaf volatiles trigger local and systemic VOC emissions in tomato
    Farag, MA
    Paré, PW
    [J]. PHYTOCHEMISTRY, 2002, 61 (05) : 545 - 554
  • [30] The lipoxygenase pathway
    Feussner, I
    Wasternack, C
    [J]. ANNUAL REVIEW OF PLANT BIOLOGY, 2002, 53 : 275 - 297