Simple detection method for distinguishing dead and living yeast colonies

被引：63

作者：

Kucsera, J

Yarita, K

Takeo, K

机构：

[1] Univ Szeged, Fac Sci, Dept Microbiol, H-6701 Szeged, Hungary

[2] Chiba Univ, Pathogen Fungi & Microbial Toxicoses Res Ctr, Dept Ultrastruct & Mol Funct, Chiba, Japan

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2000年 / 41卷 / 01期

关键词：

dead cells; dye uptake; phloxin B;

D O I：

10.1016/S0167-7012(00)00136-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and simple assay was developed for detection of yeast colonies containing dying or dead cells. Methylene blue, phloxin B, rose bengal and trypan blue at concentrations of 5-10 mu mol l(-1) were shown to stain non-viable cells in colonies of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans and Filobasidium capsuligenum without staining or affecting the viability of living cells of the colonies. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：19 / 21

页数：3

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