AMPHOTERICIN-B SUSCEPTIBILITY OF CANDIDA SPECIES ASSESSED BY RAPID FLOW CYTOMETRIC MEMBRANE-POTENTIAL ASSAY

被引：22

作者：

ORDONEZ, JV ^{[1
]}

WEHMAN, NM ^{[1
]}

机构：

[1] UNIV MARYLAND,DEPT MICROBIOL & IMMUNOL,BALTIMORE,MD 21201

来源：

CYTOMETRY | 1995年 / 22卷 / 02期

关键词：

C; ALBICANS; TROPICALIS; RAPID ANTIBIOTIC SUSCEPTIBILITY TESTING; YEAST; MEMBRANE POTENTIAL; FLOW CYTOMETRY;

D O I：

10.1002/cyto.990220213

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for detection of the susceptibility of Candida species to amphotericin B is described. The technique is a modification of a similar flow cytometric technique designed to detect antibiotic susceptibility of Staphylococcus aureus, Membrane potential changes caused by the antibiotic are measured flaw cytometrically by using the fluorescent membrane potential-sensitive dye 3,3'-dipentyloxacarbocyanine iodide. One strain each of C. albicans and C. tropicalis (both sensitive to amphotericin B) and one strain of C. tropicalis (resistant to amphotericin B) were used to validate the method. Sensitivity or resistance was easily determined after 30 min of incubation in the presence of the antibiotic, and the drug effect was evident in a dose-dependent manner. (C) 1995 Wiley-Liss, Inc.

引用

页码：154 / 157

页数：4

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