Partition of trypsin in aqueous two-phase systems of poly(ethylene glycol) and cashew-nut tree gum

被引：30

作者：

Oliveira, LA

Sarubbo, LA

Porto, ALF

Campos-Takaki, GM

Tambourgi, EB

机构：

[1] Univ Estadual Campinas, FEQ, DESQ, BR-13081 Campinas, Brazil

[2] UFPE, Lab Imunopatol Keizo Asami, Recife, PE, Brazil

[3] UFRPE, Dept Morfol & Fisiol Anim, Recife, PE, Brazil

[4] UNICAP, NPCIAMB, Dept Quim, Recife, PE, Brazil

来源：

PROCESS BIOCHEMISTRY | 2002年 / 38卷 / 05期

关键词：

aqueous two-phase system; trypsin; partition; cashew-nut tree gum; poly(ethylene glycol); enzyme activity;

D O I：

10.1016/S0032-9592(02)00191-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The partition behaviour of trypsin in poly(ethylene glycol) (PEG)-cashew-nut tree gum aqueous two-phase systems has been characterized. The enzyme partitioned preferentially into the cashew-nut tree gum phase. Investigation on the effect of the molecular weight of the PEG, the pH of system and the tie-line length of the biphasic diagram lead to the conclusion that the system properties had little effect on trypsin partition coefficients. In some cases, the NaCl addition changed dramatically the partition coefficient, this means that altering the conditions allows the manipulation of the protein partition. Maximum recovery of trypsin activity in the cashew-nut tree gum phase was obtained with PEG (molecular weight 8000) at pH 7.0 and 1.0 M NaCl. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：693 / 699

页数：7

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