Interaction of the G-protein G(11)alpha with receptors and phosphoinositidase C: The contribution of G-protein palmitoylation and membrane association

被引：18

作者：

Wise, A

Parenti, M

Milligan, G

机构：

[1] UNIV GLASGOW,INST BIOMED & LIFE SCI,DIV BIOCHEM & MOL BIOL,MOL PHARMACOL GRP,GLASGOW G12 8QQ,LANARK,SCOTLAND

[2] UNIV MILAN,DIPARTIMENTO PHARMACOL,I-20129 MILAN,ITALY

来源：

FEBS LETTERS | 1997年 / 407卷 / 03期

基金：

英国惠康基金;

关键词：

G protein; acylation; receptor; phospholipase C; inositol;

D O I：

10.1016/S0014-5793(97)00300-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Wild-type and palmitoylation defective mutants of the murine G protein G(11)alpha were transfected into HEK293 tells. Wild-type G(11)alpha was membrane associated, Cys9Ser Cys10Ser G(11)alpha was present in the soluble fraction whilst both Cys9Ser G(11)alpha and Cys10Ser G(11)alpha were distributed between the fractions. Expression of the rat TRH receptor resulted in agonist stimulation of inositol phosphate accumulation. The degree of stimulation produced by TRH following co-transfection of the palmitoylation-resistant forms of G(11)alpha compared to the wildtype protein correlated with the amount of membrane-associated G protein. (C) 1997 Federation of European Biochemical Societies.

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收藏

页码：257 / 260

页数：4

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