Liquid chromatography-ion trap tandem mass spectrometry for the characterization of polypeptide antibiotics of the colistin series in commercial samples

被引：24

作者：

Govaerts, C ^{[1
]}

Orwa, J ^{[1
]}

Van Schepdael, A ^{[1
]}

Roets, E ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Fac Farmaceut Wetenschappen, Lab Farmaceut Chem Analyse Geneesmiddelen, B-3000 Leuven, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 976卷 / 1-2期

关键词：

pharmaceutical analysis; polypeptides; antibiotics; colistins; polymyxins;

D O I：

10.1016/S0021-9673(02)00375-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A selective reversed-phase liquid chromatography-tandem mass spectrometry method is described for the characterization of related substances in the colistin complex. Mass spectral data were acquired on an LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The main advantage of this technique is the characterization of novel related substances without time-consuming isolation and purification procedures. Using this method seven new related substances were partially identified in colistin bulk sample and tablets. Four components were assigned as isomers of the main components of colistin. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：65 / 78

页数：14

共 20 条

[1] ISOLATION AND CHARACTERIZATION OF 3 NEW POLYMYXINS IN POLYMYXIN-B AND POLYMYXIN-E BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].