Identification of a toxic mechanism of the plasticizers, phtahlic acid esters, which are putative endocrine disrupters: Time-dependent increase in quinolinic acid and its metabolites in rats fed di(2-ethylhexyl)phthalate

被引:19
作者
Fukuwatari, T [1 ]
Suzuki, Y [1 ]
Sugimoto, E [1 ]
Shibata, K [1 ]
机构
[1] Univ Shiga Prefecture, Sch Human Cultures, Dept Life Style Studies, Lab Food Sci & Nutr, Shiga 5228533, Japan
关键词
phthalate ester; di(2-ethylhexyl)phthalate; quinolinic acid; endocrine disrupters; tryptophan metabolism;
D O I
10.1271/bbb.66.2687
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have reported that the administration of di(2-ethylhexyl)phthalate (DEHP) increased the formations of quinolinic acid (QA) and its lower metabolites on the tryptophan-niacin pathway. To discover the mechanism involved in disruption of the tryptophan-niacin pathway by DEHP, we assessed the daily urinary excretion of QA and its lower metabolites, and enzyme activities on the tryptophan-niacin pathway. Rats were fed with a niacin-free, 20% casein diet or the same diet supplemented with 0.1% DEHP or 0.043% phthalic acid and 0.067% 2-ethylhexanol added for 21 days. Feeding of DEHP increased the urinary excretions of QA and its lower metabolites in a time-dependent manner, and the increase of these excretions reached a peak at 11 days, but feeding of phthalic acid and 2-ethylhexanol had no effect. Feeding of DEHP, however, did not affect any enzyme activity including alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase (ACMSD), affecting the formation of QA, on the tryptophan-niacin pathway.
引用
收藏
页码:2687 / 2691
页数:5
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