Differential translational initiation of lbp mRNA is caused by a 5' upstream open reading frame

被引：13

作者：

Mittag, M

Eckerskorn, C

Strupat, K

Hastings, JW

机构：

[1] HARVARD UNIV,DEPT CELLULAR & MOL BIOL,CAMBRIDGE,MA 02138

[2] MAX PLANCK INST BIOCHEM,D-82152 MARTINSRIED,GERMANY

[3] DEPT MED PHYS & BIOPHYS,D-48149 MUNSTER,GERMANY

来源：

FEBS LETTERS | 1997年 / 411卷 / 2-3期

关键词：

circadian clock; translational control; Gonyaulax polyedra;

D O I：

10.1016/S0014-5793(97)00701-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of the luciferin-binding protein (LBP) from Gonyaulax polyedra is regulated by the circadian clack at the translational level, Here we report that in vitro translation of lbp mRNA results in the synthesis of two LBP variants of different sizes, which is shown to be due to translational initiation at different in-frame AUG codons on lbp mRNA, Differential initiation is caused by a small open reading frame (ORF, situated in the 5' untranslated region of lbp mRNA), which gives rise to a leaky scanning mechanism, In Gonyaulax, only one of these variants, which is produced by initiation from the first AUG of the lbp ORF, exhibits a circadian rhythm and is far more abundant during night phase. (C) 1997 Federation of European Biochemical Societies.

引用

页码：245 / 250

页数：6

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