Partial deletion of transposon Tn4560 integrated into the genome of Streptomyces tendae

被引：4

作者：

Engel, P

Lax, AR

机构：

[1] US Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, LA

[2] USDA, ARS, SRRC, New Orleans, LA 70124-4305

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1997年 / 25卷 / 03期

关键词：

D O I：

10.1046/j.1472-765X.1997.00208.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polymerase chain reaction (PCR), Southern hybridization and DNA sequencing experiments were done to determine whether all of Tn4560, a Streptomyces transposon, integrated into the genomes of three nikkomycin nonproducing mutants. A deletion of 279 bases occurred at one end of Tn4560 while present in the genome of one of the mutants.

引用

页码：225 / 228

页数：4

共 9 条

[1]

BIRCH AW, 1985, J GEN MICROBIOL, V131, P1299

[2] TN4556, A 6.8-KILOBASE-PAIR TRANSPOSABLE ELEMENT OF STREPTOMYCES-FRADIAE [J].

CHUNG, ST .

JOURNAL OF BACTERIOLOGY, 1987, 169 (10) :4436-4441

[3]

CHUNG ST, 1989, GENETICS MOL BIOL IN, P168

[4] METABOLIC PRODUCTS OF MICROORGANISMS .154. NIKKOMYCIN, A NEW INHIBITOR OF FUNGAL CHITIN SYNTHESIS [J].

DAHN, U ;

HAGENMAIER, H ;

HOHNE, H ;

KONIG, WA ;

WOLF, G ;

ZAHNER, H .

ARCHIVES OF MICROBIOLOGY, 1976, 107 (02) :143-160

[5]

ENGEL P, 1993, FEMS MICROBIOL LETT, V109, P257

[6]

Hopwood D.A., 1985, GENETIC MANIPULATION

[7] RANDOM INSERTION OF TN4560 IN STREPTOMYCES-LIVIDANS AND STREPTOMYCES-AVERMITILIS [J].

IRNICH, S ;

CULLUM, J .

BIOTECHNOLOGY LETTERS, 1993, 15 (09) :895-900

[8] TRANSPOSON TN4556 OF STREPTOMYCES-FRADIAE - NUCLEOTIDE-SEQUENCE OF THE ENDS AND THE TARGET SITES [J].

OLSON, ER ;

CHUNG, ST .

JOURNAL OF BACTERIOLOGY, 1988, 170 (04) :1955-1957

[9] NUCLEOTIDE-SEQUENCE OF STREPTOMYCES-FRADIAE TRANSPOSABLE ELEMENT TN4556 - A CLASS-II TRANSPOSON RELATED TO TN3 [J].

SIEMIENIAK, DR ;

SLIGHTOM, JL ;

CHUNG, ST .

GENE, 1990, 86 (01) :1-9

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