Structural Basis for Catalytic Activation of Thiocyanate Hydrolase Involving Metal-Ligated Cysteine Modification

被引：40

作者：

Arakawa, Takatoshi ^{[1
]}

Kawano, Yoshiaki ^{[2
]}

Katayama, Yoko ^{[3
]}

Nakayama, Hiroshi ^{[4
]}

Dohmae, Naoshi ^{[4
]}

Yohda, Masafumi ^{[1
]}

Odaka, Masafumi ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Grad Sch Technol, Dept Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan

[2] RIKEN, Harima Inst, Prot Crystallog Methodol Team, Adv Prot Crystallog Res Grp, Mikazuki, Hyogo 6795148, Japan

[3] Tokyo Univ Agr & Technol, Grad Sch Agr, Dept Environm & Nat Resource Sci, Fuchu, Tokyo 1838509, Japan

[4] RIKEN, Biomol Characterizat Team, Adv Dev & Supporting Ctr, Wako, Saitama 3510198, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 41期

关键词：

METALLOENZYME NITRILE HYDRATASE; PROTEIN-SULFENIC ACIDS; NONHEME IRON CENTER; POSTTRANSLATIONAL MODIFICATION; THIOBACILLUS-THIOPARUS; FUNCTIONAL EXPRESSION; CRYSTAL-STRUCTURE; ACTIVE-SITE; MECHANISM; CRYSTALLOGRAPHY;

D O I：

10.1021/ja903979s

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Thiocyanate hydrolase (SCNase) is a member of a family of nitrile hydratase proteins, each of which contains a unique noncorrin cobalt center with two post-translationally modified cysteine ligands, cysteine-sulfenic acid or -sulfenate (Cys-SO(H)), and cysteine-sulfininate (CYS-SO2-), respectively. We have found that a partially matured recombinant SCNase was activated during storage The crystal structures of SCNase before and after storage demonstrated that CYS-SO2 modification of gamma CyS131 proceeded to completion prior to storage, while Cys-SO(H) modification of gamma Cys133 occurred during storage SCNase activity was suppressed when gamma Cys133 was further oxidized to CYS-SO2-. The correlation between the catalytic activity and the extent of the gamma Cys133 modification indicates that the cysteine sulfenic acid modification of gamma Cys133 is of primary importance in determining the activity of SCNase.

引用

页码：14838 / 14843

页数：6

共 49 条

[1] Structure of thiocyanate hydrolase: A new nitrile hydratase family protein with a novel five-coordinate cobalt(III) center [J].