Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice

被引：469

作者：

Hittalmani, S ^{[1
]}

Parco, A ^{[1
]}

Mew, TV ^{[1
]}

Zeigler, RS ^{[1
]}

Huang, N ^{[1
]}

机构：

[1] Int Rice Res Inst, Manila 1099, Philippines

来源：

THEORETICAL AND APPLIED GENETICS | 2000年 / 100卷 / 07期

关键词：

RFLP markers; fine-mapping; blast resistance; pyramiding; marker-assisted selection;

D O I：

10.1007/s001220051395

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Three major genes (Pi1, Piz-5 and Pita) for blast resistance on chromosomes 11, 6 and 12, respectively, were fine-mapped and closely linked RFLP markers identified. New markers for Pi1 and Pita were found that were flanking the genes. The three genes were pyramided using RFLP markers. A PCR-based SAP (sequence amplified polymorphism) marker was used to identify Piz-5 in the segregating population. The plants carrying the two- and three-gene combinations that were tested for resistance to leaf blast in the Philippines and India indicated that combinations including Piz-5 have enhanced resistance than when it is present alone. The genes from the pyramided lines are at present being deployed into agronomically superior rice varieties by marker-aided selection (MAS).

引用

页码：1121 / 1128

页数：8

共 34 条

[21] Identification of a new blast resistance gene in the indica rice cultivar Kasalath using Japanese differential cultivars and isozyme markers [J].