Failure to Detect the Novel Retrovirus XMRV in Chronic Fatigue Syndrome

被引:168
作者
Erlwein, Otto [1 ]
Kaye, Steve [1 ]
McClure, Myra O. [1 ]
Weber, Jonathan [1 ]
Wills, Gillian [1 ]
Collier, David [2 ]
Wessely, Simon [3 ]
Cleare, Anthony [3 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Fac Med, Wright Fleming Inst, Jefferiss Res Trust Labs,Sect Infect Dis, London, England
[2] Kings Coll London, Inst Psychiat, Social Genet & Dev Psychiat Ctr, London WC2R 2LS, England
[3] Kings Coll London, Inst Psychiat, Dept Psychol Med, London WC2R 2LS, England
来源
PLOS ONE | 2010年 / 5卷 / 01期
关键词
RANDOMIZED CONTROLLED-TRIAL; COGNITIVE-BEHAVIOR THERAPY; PROSTATE-CANCER PATIENTS; GAMMARETROVIRUS; TUMORS; SCALE;
D O I
10.1371/journal.pone.0008519
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: In October 2009 it was reported that 68 of 101 patients with chronic fatigue syndrome (CFS) in the US were infected with a novel gamma retrovirus, xenotropic murine leukaemia virus-related virus (XMRV), a virus previously linked to prostate cancer. This finding, if confirmed, would have a profound effect on the understanding and treatment of an incapacitating disease affecting millions worldwide. We have investigated CFS sufferers in the UK to determine if they are carriers of XMRV. Methodology: Patients in our CFS cohort had undergone medical screening to exclude detectable organic illness and met the CDC criteria for CFS. DNA extracted from blood samples of 186 CFS patients were screened for XMRV provirus and for the closely related murine leukaemia virus by nested PCR using specific oligonucleotide primers. To control for the integrity of the DNA, the cellular beta-globin gene was amplified. Negative controls (water) and a positive control (XMRV infectious molecular clone DNA) were included. While the beta-globin gene was amplified in all 186 samples, neither XMRV nor MLV sequences were detected. Conclusion: XMRV or MLV sequences were not amplified from DNA originating from CFS patients in the UK. Although we found no evidence that XMRV is associated with CFS in the UK, this may be a result of population differences between North America and Europe regarding the general prevalence of XMRV infection, and might also explain the fact that two US groups found XMRV in prostate cancer tissue, while two European studies did not.
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