Sensitive method for the determination of ambroxol in body fluids by capillary electrophoresis and fluorescence detection

被引：35

作者：

Perez-Ruiz, T ^{[1
]}

Martínez-Lozano, C ^{[1
]}

Sanz, A ^{[1
]}

Bravo, E ^{[1
]}

机构：

[1] Univ Murcia, Fac Chem, Dept Analyt Chem, E-30071 Murcia, Spain

来源：

JOURNAL OF CHROMATOGRAPHY B | 2000年 / 742卷 / 01期

关键词：

ambroxol; capillary electrophoresis; laser-induced fluorescence; body fluids; pharmaceuticals;

D O I：

10.1016/S0378-4347(00)00150-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and rapid capillary electrophoretic method combined with laser-induced fluorescence detection has been developed for the determination of ambroxol. Samples were derivatized with 5.10(-4) M fluorescein isothiocyanate. A linear relationship between concentration and peak area was obtained in the concentration range 0.008-42 mu g ml(-1) with a correlation coefficient of 0.9999. The applicability of the method to serum and urine samples was demonstrated. The method is also useful for the determination of ambroxol in pharmaceutical preparations. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：205 / 210

页数：6

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