A simplification of the protein assay method of Ramsay et al. for the quantification of Thiobacillus ferrooxidans in the presence of ferric precipitates

被引：4

作者：

Mesa, MM ^{[1
]}

Macías, M ^{[1
]}

Cantero, D ^{[1
]}

机构：

[1] Univ Cadiz, Dept Chem Engn Food Technol & Environm Technol, Cadiz 11510, Spain

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2000年 / 53卷 / 06期

关键词：

D O I：

10.1007/s002530000321

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A variation on Ramsay's method for microbial protein determination has been developed in order to quantify Thiobacillus ferrooxictans attached to ferric precipitates or in aqueous suspensions containing such precipitates. Some modifications have been introduced to provide a method that is more sensitive, simple and rapid. A linear standard curve is presented to permit a direct correlation between the protein concentration (mg/l) and the cell concentration (10(6) cells/ml). An application of this method has been demonstrated in the quantification of biomass immobilized on the surface of polyurethane foam particles in a packed bed reactor, several experiments having been conducted to establish the best conditions for the quantification studies.

引用

页码：722 / 725

页数：4

共 14 条

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