Electrochemical detection of Cecropin CM4 gene by single stranded probe and cysteine modified gold electrode

被引：8

作者：

Fan, CH

Li, GX ^{[1
]}

Gu, QR

Zhu, JQ

Zhu, DX

机构：

[1] Nanjing Univ, Dept Biochem, Nanjing 210093, Peoples R China

[2] Nanjing Univ, Natl Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China

[3] Nanjing Univ, Dept Chem, Nanjing 210093, Peoples R China

[4] Nanjing Univ, Dept Biol & Biol Technol, Nanjing 210093, Peoples R China

来源：

ANALYTICAL LETTERS | 2000年 / 33卷 / 08期

关键词：

DNA hybridization; CM4; gene; modified electrode;

D O I：

10.1080/00032710008543137

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A single stranded Cecropin CM4 gene (108 bases) was further immobilized at a cysteine modified gold electrode with the help of water soluble 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC). The ssDNA at the modified electrode could undergo hybridization in a hybridization buffer containing single stranded plasmid pLCM-4SN. A DNA minor groove binder, Hoechst 33258, was employed to discriminate between ssDNA and dsDNA. The anodic waves in differential pulse voltammograms (DPVs), of Hoechst 33258 bound to the DNAs, were used as the indicator. This assay procedure was shown to be rapid, sensitive and precise, thus a kind of prototype DNA biosensor was developed.

引用

页码：1479 / 1490

页数：12