A cluster of mutations disrupt the avirulence but not the virulence function of AvrPto

被引:95
作者
Shan, LB [1 ]
He, P [1 ]
Zhou, JM [1 ]
Tang, XY [1 ]
机构
[1] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA
关键词
D O I
10.1094/MPMI.2000.13.6.592
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
avrPto in Pseudomonas syringae pv. tomato encodes an avirulence protein that triggers race-specific resistance in tomato plants carrying Pto. The AvrPto protein is secreted from P. syringae pv. tomato to plant cells through the type III secretion pathway and activates race-specific resistance by a direct interaction with the Pto protein, Here we report that avrPto enhances the virulence of P. syringae pv. tomato in a strain-dependent manner in tomato plants lacking Pto. To determine whether the virulence function can be structurally separated from the avirulence function, we examined the virulence activity of a group of AvrPto mutants that carry single amino acid substitutions and lack the avirulence activity on tomato plants. Three mutants that were clustered in the center of AvrPto exhibited virulence activity in tomato plants with or without Pro. The rest of the mutations abolished the virulence. The identification of these mutants suggested that the avirulence function of AvrPto can be structurally separated from the virulence function.
引用
收藏
页码:592 / 598
页数:7
相关论文
共 23 条
[1]   Determinants of pathogenicity and avirulence in plant pathogenic bacteria [J].
Collmer, A .
CURRENT OPINION IN PLANT BIOLOGY, 1998, 1 (04) :329-335
[2]   EUKARYOTIC PROTEINS EXPRESSED IN ESCHERICHIA-COLI - AN IMPROVED THROMBIN CLEAVAGE AND PURIFICATION PROCEDURE OF FUSION PROTEINS WITH GLUTATHIONE-S-TRANSFERASE [J].
GUAN, KL ;
DIXON, JE .
ANALYTICAL BIOCHEMISTRY, 1991, 192 (02) :262-267
[3]   A cloned Erwinia chrysanthemi Hrp (type III protein secretion) system functions in Escherichia coli to deliver Pseudomonas syringae Avr signals to plant cells and to secrete Avr proteins in culture [J].
Ham, JH ;
Bauer, DW ;
Fouts, DE ;
Collmer, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (17) :10206-10211
[4]   BACTERIAL-BLIGHT OF SOYBEAN - REGULATION OF A PATHOGEN GENE DETERMINING HOST CULTIVAR SPECIFICITY [J].
HUYNH, TV ;
DAHLBECK, D ;
STASKAWICZ, BJ .
SCIENCE, 1989, 245 (4924) :1374-1377
[5]   WIDESPREAD DISTRIBUTION AND FITNESS CONTRIBUTION OF XANTHOMONAS-CAMPESTRIS AVIRULENCE GENE AVRBS2 [J].
KEARNEY, B ;
STASKAWICZ, BJ .
NATURE, 1990, 346 (6282) :385-386
[6]   IMPROVED BROAD-HOST-RANGE PLASMIDS FOR DNA CLONING IN GRAM-NEGATIVE BACTERIA [J].
KEEN, NT ;
TAMAKI, S ;
KOBAYASHI, D ;
TROLLINGER, D .
GENE, 1988, 70 (01) :191-197
[7]   Successful search for a resistance gene in tomato targeted against a virulence factor of a fungal pathogen [J].
Laugé, R ;
Joosten, MHAJ ;
Haanstra, JPW ;
Goodwin, PH ;
Lindhout, P ;
De Wit, PJGM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (15) :9014-9018
[8]   Bacterial avirulence genes [J].
Leach, JE ;
White, FF .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1996, 34 :153-179
[9]   AVRA AND AVRE IN PSEUDOMONAS-SYRINGAE PV TOMATO PT23 PLAY A ROLE IN VIRULENCE ON TOMATO PLANTS [J].
LORANG, JM ;
SHEN, H ;
KOBAYASHI, D ;
COOKSEY, D ;
KEEN, NT .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1994, 7 (04) :508-515
[10]   MAP-BASED CLONING OF A PROTEIN-KINASE GENE CONFERRING DISEASE RESISTANCE IN TOMATO [J].
MARTIN, GB ;
BROMMONSCHENKEL, SH ;
CHUNWONGSE, J ;
FRARY, A ;
GANAL, MW ;
SPIVEY, R ;
WU, TY ;
EARLE, ED ;
TANKSLEY, SD .
SCIENCE, 1993, 262 (5138) :1432-1436