Thioesterases and the premature termination of polyketide chain elongation in rifamycin B biosynthesis by Amycolatopsis mediterranei S699

被引:45
作者
Doi-Katayama, Y
Yoon, YJ
Choi, CY
Yu, TW
Floss, HG
Hutchinson, CR
机构
[1] Univ Wisconsin, Sch Pharm, Madison, WI 53706 USA
[2] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
[3] Seoul Natl Univ, Coll Engn, Div Chem Engn, Seoul, South Korea
[4] Univ Washington, Dept Chem, Seattle, WA 98195 USA
关键词
D O I
10.7164/antibiotics.53.484
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The role of two thioesterase genes in the premature release of polyketide synthase intermediates during rifamycin biosynthesis in the Amycolatopsis mediterranei S699 strain was investigated. Creation of:an in-frame deletion in the rifR gene led to a 30 similar to 60% decrease in the production of both rifamycin B by the S699 strain or a series of tetra- to decaketide shunt products of polyketide chain assembly by the rifF strain. Since a similar percentage decrease was seen in both genetic backgrounds, we conclude that the RifR thioesterase 2 is not involved in premature release of the carbon chain assembly intermediates. Similarly, fusion of the Saccharopolyspora erythraea DEBS3 thioesterase 1 domain to the C-terminus of the RifE PKS subunit did not result in a noticeable increase in the amount of the undecaketide intermediate formed nor in the amounts of the tetra- to decaketide shunt products. Hence, premature release of the carbon chain assembly intermediates is an unusual property of the Rif PKS itself.
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页码:484 / 495
页数:12
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