A comparison study on microwave-assisted extraction of Artemisia sphaerocephala polysaccharides with conventional method: Molecule structure and antioxidant activities evaluation

被引：160

作者：

Wang, Junlong ^{[1
,2
]}

Zhang, Ji ^{[1
,2
]}

Wang, Xiaofang ^{[1
,2
]}

Zhao, Baotang ^{[1
,2
]}

Wu, Yiqian ^{[1
,2
]}

Yao, Jian ^{[1
,2
]}

机构：

[1] NW Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China

[2] NW Normal Univ, Bioact Prod Engn Res Ctr Gansu Distinct Plants, Lanzhou 730070, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2009年 / 45卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Artemisia sphaerocephala polysaccharides; Microwave-assisted extraction; Box-Behnken design; Chain conformation; Antioxidant; CHEMICAL-CHARACTERIZATION; CRUDE POLYSACCHARIDES; IN-VITRO; OPTIMIZATION; PURIFICATION; FRACTIONS; DERIVATIVES; ANTITUMOR; MUSHROOM;

D O I：

10.1016/j.ijbiomac.2009.09.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The conventional extraction methods for polysaccharides were time-consuming, laborious and energy-consuming. Microwave-assisted extraction (MAE) technique was employed for the extraction of Artemisia sphaerocephala polysaccharides (ASP),which is a traditional Chinese food. The extracting parameters were optimized by Box-Behnken design. In microwave heating process, a decrease in molecular weight (M-w) was detected in SEC-LLS measurement. A d(f) value of 2.85 indicated ASP using MAE exhibited as a sphere conformation of branched clusters in aqueous solution. Furthermore, it showed stronger antioxidant activities compared with hot water extraction. The data obtained showed that the molecular weights played a more important role in antioxidant activities. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.

引用

页码：483 / 492

页数：10

共 33 条

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Antioxidant activities of different fractions of polysaccharide conjugates from green tea (Camellia Sinensis) [J].