Role of the prohormone convertase PC2 in the processing of proglucagon to glucagon

被引：55

作者：

Rouille, Y ^{[1
]}

Bianchi, M ^{[1
]}

Irminger, JC ^{[1
]}

Halban, PA ^{[1
]}

机构：

[1] CTR MED UNIV GENEVA,LABS RECH LOUIS JEANTET,CH-1211 GENEVA 4,SWITZERLAND

来源：

FEBS LETTERS | 1997年 / 413卷 / 01期

关键词：

proglucagon; prohormone processing; islet of Langerhans; adenovirus vector; alpha-cell;

D O I：

10.1016/S0014-5793(97)00892-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proglucagon is alternatively processed to glucagon in pancreatic alpha-cells, or to glucagon-like peptide-1 in intestinal L cells. Here, the specificity of PC2, the major prohormone convertase of alpha-cells, was examined both in vivo and in vitro. Adenovirus-mediated co-expression of proglucagon and PC2 in GH(4)C(1) cells resulted in a pattern of processing products very similar to that observed in alpha-cells. Oxyntomodulin, an intermediate in the processing of proglucagon, was quantitatively converted to glucagon in vitro by purified recombinant PC2, in combination with carboxypeptidase E. It is concluded that PC2 is able to act alone in the pancreatic pathway of proglucagon processing. (C) 1997 Federation of European Biochemical Societies.

引用

页码：119 / 123

页数：5

共 51 条

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