Presence of melanocortin (MC4) receptor in spiny dogfish suggests an ancient vertebrate origin of central melanocortin system

被引:53
作者
Ringholm, A
Klovins, J
Fredriksson, R
Poliakova, N
Larson, ET
Kukkonen, JP
Larhammar, D
Schiöth, HB
机构
[1] Uppsala Univ, Dept Neurosci, Div Pharmacol, Biomed Ctr, S-75124 Uppsala, Sweden
[2] Uppsala Univ, Dept Neurosci, Div Physiol, Biomed Ctr, S-75124 Uppsala, Sweden
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 2003年 / 270卷 / 02期
关键词
GPCR; melanocortin; melanocyte-stimulating hormone; receptor;
D O I
10.1046/j.1432-1033.2003.03371.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report the cloning, expression, pharmacological characterization and tissue distribution of a melanocortin (MC) receptor gene in a shark, the spiny dogfish (Squalus acanthias ) (Sac). Phylogenetic analysis showed that this receptor is an ortholog of the MC4 subtype, sharing 71% overall amino acid identity with the human (Hsa) MC4 receptor. When expressed and characterized by radioligand binding assay for the natural MSH (melanocyte-stimulating hormone) peptides alpha-, beta-, and gamma-MSH, the SacMC4 receptor showed pharmacological properties very similar to the HsaMC4 receptor. Stimulation of SacMC4 receptor transfected cells with alpha-MSH caused a dose-dependent increase in intracellular cAMP levels. The SacMC4 receptor has Ala in position 59 where all other cloned MC receptors have Glu. We confirmed that this was not due to individual polymorphism and subsequently mutated the residue 'back' to Glu but the mutation did not affect the pharmacological properties of the receptor. SacMC4 receptor mRNA was detected by RT-PCR in the optic tectum, hypothalamus, brain stem, telencephalon and olfactory bulb but not in cerebellum or in peripheral tissues. This study describes the first characterization of an MC receptor in a cartilaginous fish, the most distant MC receptor gene cloned to date. Conservation of gene structure, pharmacological properties and tissue distribution suggests that this receptor may have similar roles in sharks as in mammals and that these were established more than 450 million years ago.
引用
收藏
页码:213 / 221
页数:9
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