The use of RNA probes for the analysis of gene expression

被引：8

作者：

Belin, D ^{[1
]}

机构：

[1] UNIV GENEVA, SCH MED, DEPT PATHOL, CMU, CH-1211 GENEVA, SWITZERLAND

来源：

MOLECULAR BIOTECHNOLOGY | 1997年 / 7卷 / 02期

关键词：

RNA; riboprobe; phage; RNA polymerase; transcription; hybridization;

D O I：

10.1007/BF02761751

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The monomeric bacteriophage RNA polymerases allow the synthesis of virtually any RNA molecule in unlimited quantity. In this protocol, we describe the preparation of plasmid and PCR-derived templates. A basic transcription protocol is provided with several optional modifications. The use of RNA probes in Northern blot hybridization and in RNase protection assays is described. The relative advantages and pitfalls of these two methods to quantitatively detect mRNA targets are discussed.

引用

收藏

页码：153 / 163

页数：11

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