Acetylcholine biosensor involving entrapment of acetylcholinesterase and poly(ethylene glycol)-modified choline oxidase in a poly(vinyl alcohol) cryogel membrane

被引：80

作者：

Doretti, L

Ferrara, D

Lora, S

Schiavon, F

Veronese, FM

机构：

[1] CNR, Serv Prevenz & Protez, I-35127 Padua, Italy

[2] CNR, Ist Fotochim & Radiaz Alta Energia, I-35020 Legnaro, Padova, Italy

[3] Univ Padua, Dipartimento Sci Farmaceut, I-35131 Padua, Italy

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2000年 / 27卷 / 3-5期

关键词：

biosensor; choline sensor; acetylcholine sensor; PEG-modified choline oxidase; PVA cryogel;

D O I：

10.1016/S0141-0229(00)00210-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A bienzymatic sensor for the determination of acetylcholine was prepared by physical coimmobilization of acetylcholinesterase and poly(ethylene glycol)-modified choline oxidase in a poly(vinyl alcohol) cryogel membrane obtained by a cyclic freezing-thawing process. The enzyme-modified polymer was applied on a platinum electrode to form an amperometric sensor, based on the electrochemical detection of enzymatically developed hydrogen peroxide. The analytical characteristics of this sensor, including calibration curves for choline and acetylcholine, pH, and temperature effects, and stability an described. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：279 / 285

页数：7

共 21 条

[1] AMPEROMETRIC ENZYME ELECTRODES .3. A CONDUCTING SALT ELECTRODE FOR THE OXIDATION OF 4 DIFFERENT FLAVOENZYMES [J].