High Interlaboratory Reproducibility of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Species Identification of Nonfermenting Bacteria

被引：132

作者：

Mellmann, A. ^{[1
]}

Bimet, F. ^{[2
]}

Bizet, C. ^{[2
]}

Borovskaya, A. D. ^{[3
]}

Drake, R. R. ^{[4
]}

Eigner, U. ^{[5
]}

Fahr, A. M. ^{[5
]}

He, Y. ^{[6
,7
]}

Ilina, E. N. ^{[3
]}

Kostrzewa, M. ^{[8
]}

Maier, T. ^{[8
]}

Mancinelli, L. ^{[9
]}

Moussaoui, W. ^{[10
]}

Prevost, G. ^{[10
]}

Putignani, L. ^{[9
]}

Seachord, C. L. ^{[4
]}

Tang, Y. W. ^{[6
,7
]}

Harmsen, D. ^{[11
]}

机构：

[1] Univ Klinikum Munster, Inst Hyg, D-48149 Munster, Germany

[2] Inst Pasteur, Ctr Ressources Biol, Dept Microbiol, F-75724 Paris, France

[3] Res Inst Phys Chem Med, Moscow 119992, Russia

[4] Eastern Virginia Med Sch, Dept Microbiol & Mol Cell Biol, Ctr Biomed Prote, Norfolk, VA 23501 USA

[5] Labor Limbach, D-69126 Heidelberg, Germany

[6] Vanderbilt Univ, Sch Med, Dept Med, Nashville, TN 37232 USA

[7] Vanderbilt Univ, Sch Med, Dept Pathol, Nashville, TN 37232 USA

[8] Bruker Daltonik GmbH, Bremen, Germany

[9] Bambino Gesu Pediat Hosp, Microbiol Unit, Hlth Care & Res Inst, I-00165 Rome, Italy

[10] Inst Bacteriol UR Physiopathol & Med Translat, F-67000 Strasbourg, France

[11] Univ Hosp Muenster, Dept Periodontol, D-48149 Munster, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 11期

关键词：

INTACT MICROORGANISMS; RAPID IDENTIFICATION; DESORPTION/IONIZATION;

D O I：

10.1128/JCM.00921-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Matrix-assisted laser desorption ionization-time of flight mass spectrometry has emerged as a rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study ( using eight laboratories) achieved 98.75% interlaboratory reproducibility. Only 6 of the 480 samples were misidentified due to interchanges ( 4 samples) or contamination ( 1 sample) or not identified because of insufficient signal intensity ( 1 sample).

引用

页码：3732 / 3734

页数：3

共 13 条

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