Telomerase activity is elevated in early S phase in hamster cells

Telomerase is a ribonucleoprotein that elongates telomeric repeats de novo. We examined the possibility that telomerase activity is cell cycle regulated by examining telomerase activity in cell cycle synchronized Chinese hamster ovary (CHO) B11 cells. Overall telomerase activity was similar in growing and quiescent cells. Further, cells synchronized in G(1), S, or G(2)/M showed similar levels of telomerase activity. However, a detailed analysis of cells within S phase showed that there was a higher level of telomerase activity in early S phase when compared with other points in the cell cycle, These results suggest a relationship between telomerase activity and cell cycle regulation. (C) 1997 Academic Press.