Mitochondria Targeted Nanoscale Zeolitic Imidazole Framework-90 for ATP Imaging in Live Cells

被引：431

作者：

Deng, Jingjing ^{[1
]}

Wang, Kai ^{[1
,2
]}

Wang, Ming ^{[1
,2
]}

Yu, Ping ^{[1
,2
]}

Mao, Lanqun ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2017年 / 139卷 / 16期

基金：

美国国家科学基金会;

关键词：

METAL-ORGANIC FRAMEWORKS; PHOSPHATE-CONTAINING METABOLITES; CONTROLLED DRUG-DELIVERY; FORMALDEHYDE GAS SENSOR; CARBON-DIOXIDE CAPTURE; ONE-POT SYNTHESIS; ADENOSINE-TRIPHOSPHATE; LIVING CELLS; MOLECULAR RECOGNITION; NANOPARTICLES;

D O I：

10.1021/jacs.7b01229

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Zeolitic imidazole frameworks (ZIFs) are an emerging class of functional porous materials with promising biomedical applications such as molecular sensing and intracellular drug delivery. We report herein the first example of using nanoscale ZIFs (i.e., ZIF-90), self-assembled from Zn2+ and imidazole-2-carboxyaldehyde, to target subcellular mitochondria and image dynamics of mitochondrial ATP in live cells. Encapsulation of fluorescent Rhodamine B (RhB) into ZIF-90 suppresses the emission of RhB, while the competitive coordination between ATP and the metal node of ZIF-90 dissembles ZIFs, resulting in the release of RhB for ATP sensing. With this method, we are able to image mitochondrial ATP in live cells and study the ATP level fluctuation in cellular glycolysis and apoptosis processes. The strategy reported here could be further extended to tune nanoscale ZIFs inside live cells for targeted delivery of therapeutics to subcellular organelles for advanced biomedical applications.

引用

页码：5877 / 5882

页数：6

共 53 条

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Zeolitic Imidazole Framework (ZIF) Nanospheres for Easy Encapsulation and Controlled Release of an Anticancer Drug Doxorubicin under Different External Stimuli: A Way toward Smart Drug Delivery System [J].