Evaluation of two live attenuated cold-adapted H5N1 influenza virus vaccines in healthy adults

被引：100

作者：

Karron, Ruth A. ^{[1
]}

Talaat, Kawsar ^{[1
]}

Luke, Catherine ^{[2
]}

Callahan, Karen ^{[1
]}

Thumar, Bhagvanji ^{[1
]}

DiLorenzo, Susan ^{[1
]}

McAuliffe, Josephine ^{[2
]}

Schappell, Elizabeth ^{[1
]}

Suguitan, Amorsolo ^{[2
]}

Mills, Kimberly ^{[2
]}

Chen, Grace ^{[2
]}

Lamirande, Elaine ^{[2
]}

Coelingh, Kathleen ^{[3
]}

Jin, Hong ^{[3
]}

Murphy, Brian R. ^{[2
]}

Kemble, George ^{[3
]}

Subbarao, Kanta ^{[2
]}

机构：

[1] Johns Hopkins Bloomberg Sch Publ Hlth, Ctr Immunizat Res, Baltimore, MD 21205 USA

[2] NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA

[3] Medimmune Inc, Gaithersburg, MD 20878 USA

来源：

VACCINE | 2009年 / 27卷 / 36期

基金：

美国国家卫生研究院;

关键词：

Influenza; Vaccine; Pandemic; H5N1; Clinical trial; RANDOMIZED CONTROLLED-TRIAL; MF59-ADJUVANTED INFLUENZA; PHASE-I; ANTIBODY-RESPONSES; YOUNG-CHILDREN; SAFETY; IMMUNOGENICITY; CANDIDATE; REASSORTANT; TRIVALENT;

D O I：

10.1016/j.vaccine.2009.05.099

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background: Development of live attenuated influenza vaccines (LAIV) against avian viruses with pandemic potential is an important public health strategy. Methods and findings: We performed open-label trials to evaluate the safety, infectivity, and immunogenicity of H5N1 VN 2004 AA ca and H5N1 HK 2003 AA ca. Each of these vaccines contains a modified H5 hemagglutinin and unmodified N1 neuraminidase from the respective wild-type (wt) parent virus and the six internal protein gene segments of the A/Ann Arbor/6/60 cold-adapted (ca) master donor virus. The H5N1 VN 2004 AA ca vaccine virus was evaluated at dosages of 10(6.7) TCID50 and 10(7.5) TCID50, and the H5N1 HK 2003 AA ca vaccine was evaluated at a dosage of 10(7.5) TCID50. Two doses were administered intranasally to healthy adults in isolation at 4-8 week intervals. Vaccine safety was assessed through daily examinations and infectivity was assessed by viral culture and by realtime reverse transcription-polymerase chain reaction testing of nasal wash (NW) specimens. Immunogenicity was assessed by measuring hemagglutination-inhibition (HI) antibodies, neutralizing antibodies, and IgG or IgA antibodies to recombinant (r)H5 VN 2004 hemagglutinin (HA) in serum or NW. Fifty-nine participants were enrolled: 21 received 10(6.7) TCID50 and 21 received 10(6.7) TCID50 of H5N1 VN 2004 AA ca and 17 received H5N1 HK 2003 AA ca. Shedding of vaccine virus was minimal, as were HI and neutralizing antibody responses. Fifty-two percent of recipients of 10(7.5) TCID50 of H5N1 VN 2004 AA ca developed a serum IgA response to rH5 VN 2004 HA. Conclusions: The live attenuated H5N1 VN 2004 and HK 2003 AA ca vaccines bearing avian H5 HA antigens were very restricted in replication and were more attenuated than seasonal LAIV bearing human H1, H3 or B HA antigens. The H5N1 AA ca LAW elicited serum ELISA antibody but not HI or neutralizing antibody responses in healthy adults. (ClinicalTrials.gov Identifiers: NCT00347672 and NC700488046). (C) 2009 Published by Elsevier Ltd.

引用

页码：4953 / 4960

页数：8

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