Cloning and expression of the 11 beta-hydroxysteroid dehydrogenase type 1 gene in the baboon

被引:12
作者
Davies, WA
Luo, H
Dong, KW
Albrecht, ED
Pepe, GJ
机构
[1] EASTERN VIRGINIA MED SCH,DEPT PHYSIOL,NORFOLK,VA 23501
[2] EASTERN VIRGINIA MED SCH,DEPT OBSTET & GYNECOL,NORFOLK,VA 23501
[3] UNIV MARYLAND,SCH MED,CTR STUDIES REPROD,DEPT OBSTET GYNECOL,BALTIMORE,MD 21201
[4] UNIV MARYLAND,SCH MED,CTR STUDIES REPROD,DEPT PHYSIOL,BALTIMORE,MD 21201
基金
美国国家卫生研究院;
关键词
11; beta-HSD-1; gene; gene cloning; gene expression; transcription start site;
D O I
10.1016/S0303-7207(97)04008-2
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In the baboon, the 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD)-catalyzed metabolism of maternal cortisol and cortisone by the placenta is an important component in the sequence of events regulating the function of the fetal pituitary-adrenocortical axis. The present study was designed to isolate and sequence the promoter region of the baboon 11 beta-HSD-1 gene. The 11 beta-HSD-1 genomic DNA was isolated from a baboon kidney genomic library using a human 11 beta-HSD-1 cDNA as a probe. The sequence of a 1.7 kb fragment of the 5'-flanking region showed extensive homology (> 95) to that published by others for human 11 beta-HSD-1 particularly in exons I and II (> 95%) and in the proximal promoter (> 98%). Using total RNA from adult baboon liver annealed with a 22 bp antisense primer located at the 3' end of Exon I, parallel genomic sequencing reactions with the same primer confirmed that the baboon 11 beta-HSD-1 gene has two transcriptional start sites 93 nucleotides apart and that both start sites are preceded by a CAAT box but not a TATA box. RNase protection assays confirmed that both transcription start sites are utilized in liver and near term baboon placenta and that transcripts emanating from the downstream start site dominate in the placenta in contrast to the preferential utilization of the upstream start site in adult liver. (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码:201 / 209
页数:9
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