Production and separation of the methionine rich fraction from chick pea protein hydrolysate generated by proteases of Bacillus amyloliquefaciens

被引：11

作者：

George, S

Sivasankar, B

Jayaraman, K

Vijayalakshmi, MA

机构：

[1] ANNA UNIV, CTR BIOTECHNOL, MADRAS 600025, TAMIL NADU, INDIA

[2] UNIV TECHNOL COMPIEGNE, F-60206 COMPIEGNE, FRANCE

来源：

PROCESS BIOCHEMISTRY | 1997年 / 32卷 / 05期

关键词：

Bacillus amyloliquefaciens; solid substrate fermentation; hydrolysate; immobilised metal ion affinity chromatography; methionine;

D O I：

10.1016/S0032-9592(96)00082-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crude proteases produced by Bacillus amyloliquefaciens in solid substrate fermentation (SSF) were utilised for the production of a protein hydrolysate using chick pea as the protein source. These enzymes were found to be better hydrolytic agents than fungal enzymes. The hydrolysate generated was separated on an immobilised metal ion affinity chromatography (Cu2+) column using a volatile elution buffer. Two fractions were rich in the amino acid methionine and could be used as a peptide fortifier in different food products. (C) 1997 Elsevier Science Ltd.

引用

页码：401 / 404

页数：4

共 10 条

[1]

DEBOER AS, 1991, APPL MICROBIOL BIOT, V36, P1

[2]

*FAO, 1970, FAO TECHN REP 24

[3] PRODUCTION OF PROTEASE BY BACILLUS-AMYLOLIQUEFACIENS IN SOLID-STATE FERMENTATION AND ITS APPLICATION IN THE UNHAIRING OF HIDES AND SKINS [J].