Pyrosequencing of the DNA gyrase gene in Neisseria species:: effective indicator of ciprofloxacin resistance in Neisseria gonorrhoeae

被引：9

作者：

Lindback, Emma ^{[1
]}

Unemo, Magnus

Akhras, Michael

Gharizadeh, Baback

Fredlund, Hans

Pourmand, Nader

Wretlind, Bengt

机构：

[1] Karolinska Univ Hosp, Dept Clin Bacteriol, Div Lab Med, Huddinge, Sweden

[2] Orebro Univ Hosp, Dept Clin Microbiol, Natl Reference Lab Pathogen Neisseria, Orebro, Sweden

[3] Stanford Univ, Stanford Genome Technol Ctr, Stanford, CA 94305 USA

来源：

APMIS | 2006年 / 114卷 / 12期

关键词：

Neisseria gonorrhoeae; Neisseria species; ciprofloxacin; gyrA; species verification;

D O I：

10.1111/j.1600-0463.2006.apm_495.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The quinolone resistance determining region (QRDR) of the gyrA gene in ciprofloxacin-susceptible strains (n=53) and strains of Neisseria spp. with reduced susceptibility (n=70) was determined by the pyrosequencing method. Results showed that the QRDR of the gyrA gene is an effective molecular indicator of resistance to ciprofloxacin in Neisseria gonorrhoeae, and presumably in Neisseria meningitidis, but not in all other Neisseria spp. This sequence was not unique for N. gonorrhoeae and seems unsuitable for species verification of N. gonorrhoeae. However, whether it is also possible to use this region for verification depends on the specificity of the primary screening method used.

引用

页码：837 / 841

页数：5

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