Immunoassays for aflatoxins

被引:218
作者
Li, Peiwu [1 ]
Zhang, Qi [1 ]
Zhang, Wen [1 ]
机构
[1] Chinese Acad Agr Sci, Oil Crops Res Inst, Dept Qual Stand & Food Safety, Wuhan 430062, Peoples R China
基金
美国国家科学基金会;
关键词
Aflatoxin; Agricultural product; Antibody; Hapten; Immunoassay; Immunogen; Immunosensor; Nanoparticle label; Non-competitive assay; MOLECULARLY IMPRINTED POLYMERS; LINKED-IMMUNOSORBENT-ASSAY; ELECTROCHEMICAL IMMUNOSENSOR; MONOCLONAL-ANTIBODIES; ENZYME-IMMUNOASSAY; COMPETITIVE ELISA; M1; DETERMINATION; RAPID DETECTION; QUANTUM DOTS; OCHRATOXIN-A;
D O I
10.1016/j.trac.2009.07.003
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Immunoassays for aflatoxin analysis have been regarded as valuable supplements to existing and rapidly developing chromatographic techniques. We describe six types of aflatoxin immunogens and their characteristics, reported antibodies against aflatoxins, traditional and novel labeled materials for assay signaling, three immunoassay formats, assay devices (e.g., microtiter plate and reader, lateral flow strip, electronic and optical immunosensors, and a rapid tester dedicated to aflatoxins) and applications of immunoassay in agricultural products. We show trends towards sensitivity, simplification, intelligence and portability. After setting out five challenges in developing immunoassays, we predict that techniques involving novel nanoparticle labels and non-competitive assay may become the main trends in research and that immunoassay devices will be used in many fields. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1115 / 1126
页数:12
相关论文
共 84 条
[61]   Simultaneous enzyme immunoassay for the screening of aflatoxin B1 and ochratoxin A in chili samples [J].
Saha, Debjani ;
Acharya, Debopam ;
Roy, Dipika ;
Shrestha, Dilip ;
Dhar, Tarun K. .
ANALYTICA CHIMICA ACTA, 2007, 584 (02) :343-349
[62]   Filtration-based tyramide amplification technique-a new simple approach for rapid detection of aflatoxin B1 [J].
Saha, Debjani ;
Acharya, Debopam ;
Roy, Dipika ;
Dhar, Tarun K. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 387 (03) :1121-1130
[63]   Aflatoxin contents of stored and artificially inoculated cereals and nuts [J].
Saleemullah ;
Iqbal, A ;
Khalil, IA ;
Shah, H .
FOOD CHEMISTRY, 2006, 98 (04) :699-703
[64]   Indirect competitive immunoassay for detection of aflatoxin B1 in corn and nut products using the array biosensor [J].
Sapsford, Kim E. ;
Taitt, Chris R. ;
Fertig, Stephanie ;
Moore, Martin H. ;
Lassman, Michael E. ;
Maragos, Chris M. ;
Shriver-Lake, Lisa C. .
BIOSENSORS & BIOELECTRONICS, 2006, 21 (12) :2298-2305
[65]  
SAUNDERS GC, 1985, CLIN CHEM, V31, P2020
[66]   Rapid methods for deoxynivalenol and other trichothecenes [J].
Schneider, E ;
Curtui, V ;
Seidler, C ;
Dietrich, R ;
Usleber, E ;
Märtlbauer, E .
TOXICOLOGY LETTERS, 2004, 153 (01) :113-121
[67]   Qualitative and quantitative detection of aflatoxin B-1 in poultry sera by enzyme-linked immunosorbent assay [J].
Sekhon, CPS ;
Kapur, J ;
Sodhi, SS ;
Jand, SK .
JOURNAL OF BIOSCIENCES, 1996, 21 (04) :471-476
[68]   Complex target SELEX [J].
Shamah, Steven M. ;
Healy, Judith M. ;
Cload, Sharon T. .
ACCOUNTS OF CHEMICAL RESEARCH, 2008, 41 (01) :130-138
[69]   Synthesis of haptens and conjugates for ELISA of glycitein: Development and validation of an immunological test [J].
Shinkaruk, Svitlana ;
Lamothe, Valerie ;
Schmitter, Jean-Marie ;
Fructus, Aurelie ;
Sauvant, Patrick ;
Vergne, Sebastien ;
Degueil, Marie ;
Babin, Pierre ;
Bennetau, Bernard ;
Bennetau-Pelissero, Catherine .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (16) :6809-6817
[70]   Development of a portable field immunoassay for the detection of aflatoxin M1 in milk [J].
Sibanda, L ;
De Saeger, S ;
Van Peteghem, C .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1999, 48 (03) :203-209