A plasma polymerized film for surface plasmon resonance immunosensing

We propose a novel ethylenediamine plasma-polymerized film matrix that is deposited on gold surfaces for use in surface plasmon resonance immunosensing. The films formed on the gold surfaces are extremely thin (similar to 100 nm), are homogeneous, demonstrate good adhesion, have a fiat profile, and incorporate amino groups to introduce a chemical functionality. A sensor chip made with the film has many advantages when compared with conventionally used designs such as carboxylated dextran hydrogels. For example, a sensor chip made with the film shows a better sensor response than a conventional design partly because antibodies are densely and two-dimensionally immobilized onto the surface of the plasma-polymerized film.