Liquid chromatography method for the analysis of adenosine compounds

被引：46

作者：

Haink, G ^{[1
]}

Deussen, A ^{[1
]}

机构：

[1] Tech Univ Dresden, Med Fak Carl Gustav Carus, Inst Physiol, D-01307 Dresden, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 784卷 / 01期

关键词：

adenosine compounds;

D O I：

10.1016/S1570-0232(02)00752-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A newly available chromatography column packing material that employs hybrid particle technology was used to improve the analysis of adenosine compounds. Using a TBAS buffer/acetonitrile gradient this material permits separation of etheno-adenosine compounds in less than 4 min with excellent resolution and sensitivity (50 fmol). Variability of compound quantification is small (coefficients of variation 0.23 +/- 0.14% for 50 pmol and 1.70 +/- 0.53% for 0.5 pmol). The new method is well suited for the analysis of adenosine compounds in small biological samples and permits a high sample throughput in autosampler setups with high precision and reproducibility. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：189 / 193

页数：5

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