Studying Cilia in Zebrafish

被引：14

作者：

Drummond, Iain ^{[1
,2
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机构：

[1] Harvard Univ, Sch Med, Dept Med, Charlestown, MA 02129 USA

[2] Harvard Univ, Sch Med, Dept Genet, Charlestown, MA 02129 USA

[3] Massachusetts Gen Hosp, Charlestown, MA 02129 USA

来源：

CILIA: MODEL ORGANISMS AND INTRAFLAGELLAR TRANSPORT | 2009年 / 93卷

关键词：

MUTATIONS AFFECTING DEVELOPMENT; POLYCYSTIC KIDNEY-DISEASE; ZINC-FINGER NUCLEASES; INTRAFLAGELLAR TRANSPORT; KUPFFERS VESICLE; PRONEPHROS; GENES; DIFFERENTIATION; MOTILITY; BRAIN;

D O I：

10.1016/S0091-679X(08)93011-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Zebrafish are ideally suited for analysis of genes required for ciliogenesis and cilia function. Combining genetic manipulation with high-quality in vivo imaging, zebrafish embryos provide a high-throughput system for annotation of the cilia proteome. The specific advantages of the system are the availability of cilia mutants, the ability to target genes of unknown function using antisense methods, the feasibility of observing cilia in living embryos, and the ability to image fixed cilia in wholemount at high resolution. Techniques are described for analysis of mutants, gene knockdown using antisense morpholino oligos, visualizing cilia and cilia orientation in wholemount zebrafish embryos, cilia imaging by high-speed video, and electron microscopy of zebrafish cilia.

引用

页码：197 / +

页数：22

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