Taenia saginata derived synthetic peptides with potential for the diagnosis of bovine cysticercosis

被引:20
作者
Ferrer, E
Benitez, L
Foster-Cuevas, M
Bryce, D
Wamae, LW
Onyango-Abuje, JA
Garate, T
Harrison, LJS [1 ]
Parkhouse, RME
机构
[1] Univ Edinburgh, Easter Bush Vet Ctr, Sir Alexander Robertson Ctr Trop Vet Med, Dept Trop Anim Hlth, Roslin EH25 9RG, Midlothian, Scotland
[2] AFRC, Inst Anim Hlth, Pirbright Lab, Woking GU24 ONF, Surrey, England
[3] Inst Salud Carlos III, Ctr Nacl Microbiol, Madrid 28220, Spain
[4] Kenya Agr Res Inst, Natl Vet Res Ctr, Kikuyu, Kenya
[5] Gulbenkian Inst Sci, Oeiras, Portugal
关键词
Taenia saginata; oncosphere antigen; tegumental antigen; surface; synthetic peptides; ELISA; diagnosis; immunoprophylaxis;
D O I
10.1016/S0304-4017(02)00327-8
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Immunity in Taeniids is predominantly antibody mediated and thus many serological immunodeterminants will have potential in both protection and diagnosis. The antigenicity of six peptides derived from four potentially protective molecules cloned from a Taenia saginata oncospheres cDNA library have been evaluated as targets for the specific diagnosis of bovine cysticercosis. The six peptides consist of: two peptides (HP6-2 and HP6-3) derived from the sequence of the 18 kDa surface/secreted oncospheral adhesion antigen identified by McAb-HP6, two peptides (Ts45W-1 and Ts45W-5) derived from the sequence of the T. saginata homologue of the T. ovis 45W protective gene family, one peptide (TS45S-10) derived from a T. saginata sequence with significant similarity to the T. ovis 45S protective antigen, and one peptide (TEG-1) derived from the sequence of the T. saginata homologue of Echinococcus spp. main surface protein. Longitudinal studies indicate that T. saginata infected cattle respond to all six peptides by 3-4 weeks post-infection and that the antibody levels remain high for at least 12 weeks post-infection. As protection against Taeniid parasites is predominantly antibody mediated, some of these six peptides may be of value as immuno-prophylactic tools and hence also in assays to determine resistance to infection with the parasite. For diagnosis, on the other hand, only three peptides (HP6-2, TEG-1 and Ts45S-10) performed with the necessary sensitivity and specificity to determine exposure to infection with T saginata, and now merit an exhaustive evaluation prior to employment as routine diagnostic tools. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:83 / 94
页数:12
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